OZONE AS AN Adjunctive Modality

Weight Reduction Study

The purpose of this study was to evaluate the effectiveness of ozone therapy as an adjunctive modality for weight reduction in grade II adult obese subjects. Subjects: thirty grade II adult obese subjects from both sexes had BMI 30-40.

They ranged in age from 25 to 45 years. They were classified randomly into two groups of equal number.

Group 1: fifteen patients received ozone therapy with diet.
Group 2: fifteen patients received diet regimen only.

Procedures: evaluation procedures in form of measurement of BMI and waist circumference pre treatment and after three months post treatment, and therapeutic procedures for group 1: ozone therapy with low caloric diet, for group 2: low caloric diet only.

The results showed a statistically significant decrease in BMI, and waist circumference in both groups, with a higher rate of reduction in ozone with diet group. Conclusion: It could be concluded that ozone therapy can be used as an adjunctive modality for weight reduction in grade II adult obese subjects.

Ozone Therapy for Weight Loss

Ozone vs. Liposuction

In the course of the most recent conference sponsored by the Ozone Forum of India, in Mumbai, I was introduced to some new techniques applying ozone therapy in beauty treatments. What interested me most, and what I have used on a number patients since then, is a process called Ozone Lipolysis for reduction of fat, mostly along the waistline, but also in other areas of the body.

The effects have been consistent and astounding.

Everyone who was treated, lost the promised 3-4 inches along the waistline after the standard 8 sessions needed for that much progress. But there are other benefits, too, because when you use ozone for weight loss, the body automatically receives the benefits akin to systemic ozone therapy applications, like: enhancement of of the collagen and elastin synthesis, thus tightening the skin doing away with stretch marks, scars and wrinkles; plus the usual benefits of ozone treatments in terms of increased energy levels, increased sense of wellbeing, reduction of aches and pains.

In short, ozone lipolysis not only makes lose the tires that may have accumulated around your waist, it also makes your skin glow and you look overall younger and more beautiful.

The use of ozonated water and rectal insufflation in patientswith intestinal dysbiosis

Rectal Insufflation and Intestinal Dysbiosis

A functional intestinal Flora, physiologically speaking, is an important indication of a healthy organism; therefore, it is an important ally for the Function of Defense. The study was con-ducted on an adult population from January 2012 to June 2013. 34individuals were considered, regardless of age and sex, of which23 females (67.6%) and 11 males (32.4%), aged between 27 and62 (average: 43.9 years). For the evaluation of abdominal pressure like pain and discomfort caused by other typical disorders of dysbiosis, a visual-analogue scale was chosen, according to theScott and Huskisson model.

The results of the study made it possible to verify - first of all- that intestinal dysbiosis is a disease with a higher incidence with respect to what clinical data does not allow to establish on the basis of subjective and objective symptoms.

In conclusion, the study confirmed the validity of the treatment with ozonized water combined with rectal insufflation of oxygen and ozone mixture.

Comparing the effect of ozonized olive oil with clotrimazole on three Candida species C. albiacans, C. glabrata, C. krusei

Ozonized Oil and Candida

One of the most important pathogenic fungi in immune compromised patients is Candida.spp. Ozone is an allotropic form of oxygen with high oxidation power; in addition it has fungicidal effects.

Considering highly prevalence of Candida infections and drug resistance, it is important to find a low cost medicine with high effects and with low adverse effects for treating these kinds of infections.

In this study, the effect of ozonated olive oil compared with clotrimazole on three species of candida (C. albicans, C. glabrata, and C. krusei) on saboraud dextrose agar (SDA) media was evaluated.Different concentrations of ozonated olive oil (166.66, 200, 233.33, 266.66, 300 mg/ml) in culture media were prepared and poured in some plates separately. Plates without ozonated olive oil were used as negative control.

Plates containing different amount of clotrimazole (1, 2, 3, 6, and 8µg/ml) were considered as positive control. After inoculation of different candida spp. in all media, the plates were incubated at 37˚C for 72 hours and observed for fungal growth in their status every 24 hours.

Our study showed that the minimum inhibitory concentration (MIC) of ozonated olive oil for Candida krusei was 166.66, and for Candida albicans and Candida glaberata were 233.33, 200 mg/ml respectively. Clotrimazole inhibited all candida species in concentration much lower than ozonated olive oil.

Considering that the ability of ozonated olive oil to inhibit candida growth in the media, authors hope that future researches will be performed based on this study and it can be a new product for topical treatment of candidiasis.

Drinking Ozonated Tri-Distilled Water Increases Helicobacter Pylori Eradication and Promotes Healing of Duodenal Ulcer Lesions

Healing of Ulcer Lesions

Objective: Ozone is a physical bactericide that has been applied to disinfect drinking water. The study aimed to determine whether ozonated tri-distilled water in combination with the standard esomeprazole-based triple therapy could increase helicobacter pylori (Hp) eradication and promote healing of duodenal ulcer lesions.

Methods: In total, 132 patients were confirmed to be Hp infection positive using a Carbon 14 (14C) urea breath test, rapid urea enzyme test and pathological examination. The patients were subsequently divided into 4 groups with 33 patients per group. Groups 1 and 3 included the patients with chronic gastritis, whereas groups 2 and 4 included the patients with duodenal ulcers. The patients from groups 1 and 3 were administered standard esomeprazole-based triple therapy, including amoxicillin 1.0 two times per day, clarithromycin 0.5 two times per day, esomeprazole 20 mg one time per day, and hydrotalcite chewable tablets 1.0 three times per day. The patients from groups 2 and 4 drank ozonated tri-distilled water in combination with standard esomeprazole-based triple therapy: 300 ml ozonated tri-distilled water twice daily, amoxicillin 1.0 twice daily, clarithromycin 0.5 twice daily, esomeprazole 20 mg once daily, and hydrotalcite chewable tablets 1.0 thrice daily. The anti-Hp treatment period lasted 2 weeks, which was followed by esomeprazole and hydrotalcite for 4 weeks for all patient groups. Gastroscopy was performed for the patients in groups 3 and 4 after 4 weeks of antiHp therapy, and a 14C urea breath test was conducted for each patient after 4 weeks of drug withdrawal.

Results: Gastroscopy indicated that 21 patients in group 3 with duodenal ulcers were in the healing stage, and 29 patients in group 4 were in the healing stage, with a significant difference between these two groups (p = 0.022). The 14C urea breath tests were negative for 26 patients with a 79% rate of Hp elimination in group 1. The rates of Hp elimination were 97% in group 2 (n = 32), 73% in group 3 (n = 24), and 94% in group 4 (n = 31). The chi-square test demonstrated a significant difference between groups 1 and 2 (p < 0.05) and between groups 3 and 4 (p < 0.05). The Hp eradication of drinking ozonated tri-distilled water in combination with standard esomeprazole-based triple therapy was significantly increased compared with the standard esomeprazole-based triple therapy

Conclusion: Drinking ozonated tri-distilled water in combination with the standard treatment substantially enhances Hp eradication and promotes healing of duodenal ulcer lesions.

Drinking Ozonated Tri-Distilled Water Increases Helicobacter Pylori Eradication and Promotes Healing of Duodenal Ulcer Lesions

Efficacy of Ozone Against Candida

Background and Purpose: Ozone is an inorganic molecule with effective antimicrobial properties. Clinical treatment of ozonated water was used for the elimination of Candida albicans, Enterococcus faecalis, endotoxins, and biofilms from root canals. In addition, its therapeutic effects for tinea pedis, ulcers, and leishmaniasis were investigated. The purpose of the present study was to evaluate the fungicidal effects of ozone on different forms of C. albicans. In addition, antifungal susceptibility profile of strains was assessed before and after exposure to ozone.

Materials and Methods: Fifty strains of C. albicans were exposed to gaseous ozone at different times. Furthermore, biofilm formation and germ tube production were evaluated when yeast suspensions were exposed to ozone. In addition, antifungal susceptibility of ozone resistant colonies was investiagted as compared to controls.

Results: Ozone was highly effective in killing C. albicans in yeast form and inhibition of germ tube formation during 210 and 180 s, respectively. Although with increasing exposure time biofilm production was considerably decreased, resistance to ozone was much higher among vaginal and nail isolates even after 60 min. All the strains were sensitive to fluconazole, caspofungin, and terbinafine pre- and post-ozone exposure. Resistance to amphotericin B was significantly enhanced after exposure to ozone.

Conclusion: Although ozone was highly effective on the yeast form of C. albicans and it can inhibit the formation of germ tubes in C. albicans, the complete removal of biofilms did not happen even after 60 min. It seems that ozone therapy induces resistance to amphotericin B.

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