OZONE AS AN Adjunctive Modality

Weight Reduction Study

The purpose of this study was to evaluate the effectiveness of ozone therapy as an adjunctive modality for weight reduction in grade II adult obese subjects. Subjects: thirty grade II adult obese subjects from both sexes had BMI 30-40.

They ranged in age from 25 to 45 years. They were classified randomly into two groups of equal number.

Group 1: fifteen patients received ozone therapy with diet.
Group 2: fifteen patients received diet regimen only.

Procedures: evaluation procedures in form of measurement of BMI and waist circumference pre treatment and after three months post treatment, and therapeutic procedures for group 1: ozone therapy with low caloric diet, for group 2: low caloric diet only.

The results showed a statistically significant decrease in BMI, and waist circumference in both groups, with a higher rate of reduction in ozone with diet group. Conclusion: It could be concluded that ozone therapy can be used as an adjunctive modality for weight reduction in grade II adult obese subjects.

Ozone Therapy for Weight Loss

Ozone vs. Liposuction

In the course of the most recent conference sponsored by the Ozone Forum of India, in Mumbai, I was introduced to some new techniques applying ozone therapy in beauty treatments. What interested me most, and what I have used on a number patients since then, is a process called Ozone Lipolysis for reduction of fat, mostly along the waistline, but also in other areas of the body.

The effects have been consistent and astounding.

Everyone who was treated, lost the promised 3-4 inches along the waistline after the standard 8 sessions needed for that much progress. But there are other benefits, too, because when you use ozone for weight loss, the body automatically receives the benefits akin to systemic ozone therapy applications, like: enhancement of of the collagen and elastin synthesis, thus tightening the skin doing away with stretch marks, scars and wrinkles; plus the usual benefits of ozone treatments in terms of increased energy levels, increased sense of wellbeing, reduction of aches and pains.

In short, ozone lipolysis not only makes lose the tires that may have accumulated around your waist, it also makes your skin glow and you look overall younger and more beautiful.

The use of ozonated water and rectal insufflation in patientswith intestinal dysbiosis

Rectal Insufflation and Intestinal Dysbiosis

A functional intestinal Flora, physiologically speaking, is an important indication of a healthy organism; therefore, it is an important ally for the Function of Defense. The study was con-ducted on an adult population from January 2012 to June 2013. 34individuals were considered, regardless of age and sex, of which23 females (67.6%) and 11 males (32.4%), aged between 27 and62 (average: 43.9 years). For the evaluation of abdominal pressure like pain and discomfort caused by other typical disorders of dysbiosis, a visual-analogue scale was chosen, according to theScott and Huskisson model.

The results of the study made it possible to verify - first of all- that intestinal dysbiosis is a disease with a higher incidence with respect to what clinical data does not allow to establish on the basis of subjective and objective symptoms.

In conclusion, the study confirmed the validity of the treatment with ozonized water combined with rectal insufflation of oxygen and ozone mixture.

Comparing the effect of ozonized olive oil with clotrimazole on three Candida species C. albiacans, C. glabrata, C. krusei

Ozonized Oil and Candida

One of the most important pathogenic fungi in immune compromised patients is Candida.spp. Ozone is an allotropic form of oxygen with high oxidation power; in addition it has fungicidal effects.

Considering highly prevalence of Candida infections and drug resistance, it is important to find a low cost medicine with high effects and with low adverse effects for treating these kinds of infections.

In this study, the effect of ozonated olive oil compared with clotrimazole on three species of candida (C. albicans, C. glabrata, and C. krusei) on saboraud dextrose agar (SDA) media was evaluated.Different concentrations of ozonated olive oil (166.66, 200, 233.33, 266.66, 300 mg/ml) in culture media were prepared and poured in some plates separately. Plates without ozonated olive oil were used as negative control.

Plates containing different amount of clotrimazole (1, 2, 3, 6, and 8µg/ml) were considered as positive control. After inoculation of different candida spp. in all media, the plates were incubated at 37˚C for 72 hours and observed for fungal growth in their status every 24 hours.

Our study showed that the minimum inhibitory concentration (MIC) of ozonated olive oil for Candida krusei was 166.66, and for Candida albicans and Candida glaberata were 233.33, 200 mg/ml respectively. Clotrimazole inhibited all candida species in concentration much lower than ozonated olive oil.

Considering that the ability of ozonated olive oil to inhibit candida growth in the media, authors hope that future researches will be performed based on this study and it can be a new product for topical treatment of candidiasis.

Drinking Ozonated Tri-Distilled Water Increases Helicobacter Pylori Eradication and Promotes Healing of Duodenal Ulcer Lesions

Healing of Ulcer Lesions

Objective: Ozone is a physical bactericide that has been applied to disinfect drinking water. The study aimed to determine whether ozonated tri-distilled water in combination with the standard esomeprazole-based triple therapy could increase helicobacter pylori (Hp) eradication and promote healing of duodenal ulcer lesions.

Methods: In total, 132 patients were confirmed to be Hp infection positive using a Carbon 14 (14C) urea breath test, rapid urea enzyme test and pathological examination. The patients were subsequently divided into 4 groups with 33 patients per group. Groups 1 and 3 included the patients with chronic gastritis, whereas groups 2 and 4 included the patients with duodenal ulcers. The patients from groups 1 and 3 were administered standard esomeprazole-based triple therapy, including amoxicillin 1.0 two times per day, clarithromycin 0.5 two times per day, esomeprazole 20 mg one time per day, and hydrotalcite chewable tablets 1.0 three times per day. The patients from groups 2 and 4 drank ozonated tri-distilled water in combination with standard esomeprazole-based triple therapy: 300 ml ozonated tri-distilled water twice daily, amoxicillin 1.0 twice daily, clarithromycin 0.5 twice daily, esomeprazole 20 mg once daily, and hydrotalcite chewable tablets 1.0 thrice daily. The anti-Hp treatment period lasted 2 weeks, which was followed by esomeprazole and hydrotalcite for 4 weeks for all patient groups. Gastroscopy was performed for the patients in groups 3 and 4 after 4 weeks of antiHp therapy, and a 14C urea breath test was conducted for each patient after 4 weeks of drug withdrawal.

Results: Gastroscopy indicated that 21 patients in group 3 with duodenal ulcers were in the healing stage, and 29 patients in group 4 were in the healing stage, with a significant difference between these two groups (p = 0.022). The 14C urea breath tests were negative for 26 patients with a 79% rate of Hp elimination in group 1. The rates of Hp elimination were 97% in group 2 (n = 32), 73% in group 3 (n = 24), and 94% in group 4 (n = 31). The chi-square test demonstrated a significant difference between groups 1 and 2 (p < 0.05) and between groups 3 and 4 (p < 0.05). The Hp eradication of drinking ozonated tri-distilled water in combination with standard esomeprazole-based triple therapy was significantly increased compared with the standard esomeprazole-based triple therapy

Conclusion: Drinking ozonated tri-distilled water in combination with the standard treatment substantially enhances Hp eradication and promotes healing of duodenal ulcer lesions.

Drinking Ozonated Tri-Distilled Water Increases Helicobacter Pylori Eradication and Promotes Healing of Duodenal Ulcer Lesions

Efficacy of Ozone Against Candida

Background and Purpose: Ozone is an inorganic molecule with effective antimicrobial properties. Clinical treatment of ozonated water was used for the elimination of Candida albicans, Enterococcus faecalis, endotoxins, and biofilms from root canals. In addition, its therapeutic effects for tinea pedis, ulcers, and leishmaniasis were investigated. The purpose of the present study was to evaluate the fungicidal effects of ozone on different forms of C. albicans. In addition, antifungal susceptibility profile of strains was assessed before and after exposure to ozone.

Materials and Methods: Fifty strains of C. albicans were exposed to gaseous ozone at different times. Furthermore, biofilm formation and germ tube production were evaluated when yeast suspensions were exposed to ozone. In addition, antifungal susceptibility of ozone resistant colonies was investiagted as compared to controls.

Results: Ozone was highly effective in killing C. albicans in yeast form and inhibition of germ tube formation during 210 and 180 s, respectively. Although with increasing exposure time biofilm production was considerably decreased, resistance to ozone was much higher among vaginal and nail isolates even after 60 min. All the strains were sensitive to fluconazole, caspofungin, and terbinafine pre- and post-ozone exposure. Resistance to amphotericin B was significantly enhanced after exposure to ozone.

Conclusion: Although ozone was highly effective on the yeast form of C. albicans and it can inhibit the formation of germ tubes in C. albicans, the complete removal of biofilms did not happen even after 60 min. It seems that ozone therapy induces resistance to amphotericin B.

Clinical evaluation of purified Shilajit on testosterone levels in healthy volunteers

Shilajit benefits for testosterone levels

Purified Shilajit, an Ayurvedicrasayana, was evaluated in healthy volunteers of age between 45 and 55 years for its effect on male androgenic hormoneviz. testosterone in a randomised, double-blind, placebo-controlled clinical study at a dose of 250 mg twice a day. Treatment with Shilajit for consecutive 90 days revealed that it has significantly (P < 0.05) increased total testosterone, free testosterone and dehydroepiandrosterone (DHEAS) compared with placebo. Gonadotropic hormones (LH and FSH) levels were well maintained.

The effects of Shilajit supplementation on fatigue-induced decreases in muscular strength and serum hydroxyproline levels

Shilajit promotes the retention of maximal muscular strength

Shilajit is a safe, fluvic mineral complex exudate that is common to Ayurvedic medicine and is composed of fulvic acids, dibenzo-α-pyrones, proteins, and minerals. The purpose of this study was to examine the effects of 8 weeks of Shilajit supplementation at 250 mg·d− 1 (low dose) and 500 mg·d− 1 (high dose) versus placebo on maximal voluntary isometric contraction (MVIC) strength, concentric peak torque, fatigue-induced percent decline in strength, and serum hydroxyproline (HYP).

The Human Skeletal Muscle Transcriptome in Response to Oral Shilajit Supplementation

Shilajit supports positive skeletal muscle adaptation

The objective of the present study (clinicaltrials.gov NCT02026414) was to observe the effects of oral supplementation of a purified and standardized Shilajit extract on skeletal muscle adaptation in adult overweight/class I obese human subjects from the U.S. population. Shilajit is a mineral pitch that oozes out of Himalayan rocks. The study design consisted of a baseline visit, followed by 8 weeks of 250 mg of oral Shilajit supplementation b.i.d., and additional 4 weeks of supplementation with exercise. At each visit, blood samples and muscle biopsies were collected for further analysis. Supplementation was well tolerated without any changes in blood glucose levels and lipid profile after 8 weeks of oral supplementation and the additional 4 weeks of oral supplementation with exercise. In addition, no changes were noted in creatine kinase and serum myoglobin levels after 8 weeks of oral supplementation and the additional 4 weeks of supplementation with exercise. Microarray analysis identified a cluster of 17 extracellular matrix (ECM)-related probe sets that were significantly upregulated in muscles following 8 weeks of oral supplementation compared with the expression at the baseline visit. This cluster included tenascin XB, decorin, myoferlin, collagen, elastin, fibrillin 1, and fibronectin 1. The differential expression of these genes was confirmed using quantitative real-time polymerase chain reaction (RT-PCR). The study provided maiden evidence that oral Shilajit supplementation in adult overweight/class I obese human subjects promoted skeletal muscle adaptation through upregulation of ECM-related genes that control muscle mechanotransduction properties, elasticity, repair, and regeneration.

Skin Transcriptome of Middle-Aged Women Supplemented With Natural Herbo-mineral Shilajit Shows Induction of Microvascular and Extracellular Matrix Mechanisms

Shilajit improves skin perfusion in women

Shilajit is a pale-brown to blackish-brown organic mineral substance available from Himalayan rocks. We demonstrated that in type I obese humans, shilajit supplementation significantly upregulated extracellular matrix (ECM)–related genes in the skeletal muscle. Such an effect was highly synergistic with exercise. The present study (clinicaltrials.gov NCT02762032) aimed to evaluate the effects of shilajit supplementation on skin gene expression profile and microperfusion in healthy adult females.

The Science Behind NMN–A Stable, Reliable NAD+Activator and Anti-Aging Molecule

The Science Behind NMN–A Reliable NAD+Activator

In June of 2018, the World Health Organization (WHO) released the 11th edition of its International Classification of Diseases, and for the first time added aging.1 The classification of aging as a disease paves the way for new research into novel therapeutics to delay or reverse age-related illnesses such as cancer, cardiovascular and metabolic disease, and neurodegeneration.2,3 Nutrient sensing systems have been an intense focus of investigation, including mTOR (the mammalian target of rapamycin) for regulating protein synthesis and cell growth; AMPK (activated protein kinase) for sensing low energy states; and sirtuins, a family of seven proteins critical to DNA expression and aging, which can only function in conjunction with NAD+ (nicotinamide adenine dinucleotide), a coenzyme present in all living cells.4

Across the kingdom of life, an increase in intracellular levels of NAD+ triggers shifts that enhance survival, including boosting energy production and upregulating cellular repair.5 In fact, the slow, ineluctable process of aging has been described as a “cascade of robustness breakdown triggered by a decrease in systemic NAD+ biosynthesis and the resultant functional defects in susceptible organs and tissues.”6 Aging is marked by epigenetic shifts, genomic instability, altered nutrient sensing ability, telomere attrition, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and dysregulated intercellular communication.7,8

By middle age, our NAD+ levels have plummeted to half that of our youth.9 Numerous studies have demonstrated that boosting NAD+ levels increases insulin sensitivity, reverses mitochondrial dysfunction, and extends lifespan.10,11 NAD+ levels can be increased by activating enzymes that stimulate synthesis of NAD+, by inhibiting an enzyme (CD38) that degrades NAD+, and by supplementing with NAD precursors, including nicotinamide riboside(NR) and nicotinamide mononucleotide (NMN).12,13 A conceptual framework called NAD World, formulated over the last decade by developmental biologist Shin-ichiro Imai, MD, PhD, of Washington University School of Medicine, posits NMN as a critical, systemic signaling molecule that maintains biological robustness of the communication network supporting NAD+.6

Resveratrol supplementation improves metabolic control in rats with induced hyperlipidemia and type 2 diabetes

Resveratrol supplementation improves metabolic control

Resveratrol was recognized as the major factor responsible for the beneficial properties of red wine. Several resveratrol-based dietary supplements are available, but their efficacy has not been sufficiently tested. This study was designed to examine the effect of resveratrol supplementation, using a commercially available product, on the metabolic status of experimental animals with induced hyperlipidemia or type 2 diabetes mellitus(T2DM). Hyperlipidemia was induced by feeding the rats a standard pellet diet supplemented with cholesterol. T2DM was induced by adding 10% fructose to drinking water and streptozotocin. Treatment with resveratrol-based supplement improved glycemic control in diabetic animals and significantly decreased serum low-density-lipoprotein (LDL) and triglyceride levels, concurrently increasing the high-density-lipoprotein (HDL) levels in animals with hyperlipidemia. Resveratrol-treated animals had improved tolerance to glucose loading. Supplementation did not induce alterations in parameters of liver and renal function. Findings indicate that commercial resveratrol supplement improves metabolic control in rats with induced hyperlipidemia and T2DM.

Health benefits of resveratrol administration

Health benefits of resveratrol administration

Resveratrol is a polyphenol that is abundant in grape skin and seeds. Food sources of resveratrol include wine, berries, and peanuts. This compound has many properties, including activity against glycation, oxidative stress, inflammation, neurodegeneration, several types of cancer, and aging. Because resveratrol is generally welltolerated, it is believed to be a promising compound in preventing many diseases, such as diabetes and its complications. Unfortunately, this compound exhibits low bioavailability and solubility. The aim of this review is to summarize the latest information on the multiple effects of resveratrol on health and the benefits of its intake, based on in vitro and in vivo studies in animals and humans.

Elucidating the Mechanism of Absorption of Fast-Acting Insulin Aspart: The Role of Niacinamide

Niacinamide promotes fast-acting insulin aspart


Fast-acting insulin aspart (faster aspart) is a novel formulation of insulin aspart containing two additional excipients: niacinamide, to increase early absorption, and L-arginine, to optimize stability. The aim of this study was to evaluate the impact of niacinamide on insulin aspart absorption and to investigate the mechanism of action underlying the accelerated absorption.


The impact of niacinamide was assessed in pharmacokinetic analyses in pigs and humans, small angle X-ray scattering experiments, trans-endothelial transport assays, vascular tension measurements, and subcutaneous blood flow imaging.


Niacinamide increased the rate of early insulin aspart absorption in pigs, and pharmacokinetic modelling revealed this effect to be most pronounced up to ~30–40 min after injection in humans. Niacinamide increased the relative monomer fraction of insulin aspart by ~35%, and the apparent permeability of insulin aspart across an endothelial cell barrier by ~27%. Niacinamide also induced a concentration-dependent vasorelaxation of porcine arteries, and increased skin perfusion in pigs.


Niacinamide mediates the acceleration of initial insulin aspart absorption, and the mechanism of action appears to be multifaceted. Niacinamide increases the initial abundance of insulin aspart monomers and transport of insulin aspart after subcutaneous administration, and also mediates a transient, local vasodilatory effect.